laforin reactions (Chem Impex International)
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laforin reactions
![Effect of treating 32P-labeled glycogen with <t>laforin</t> or glucosidases. A Glycogen was labeled by incubation with 5 <t>μM</t> <t>[β-32P]UDP-glucose</t> (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3132/pmc04843132/pmc04843132__nihms-776793-f0004.jpg)
Laforin Reactions, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00310/Uridine-5'-diphosphate+disodium+salt/pmc04843132-160-6-10
Average 95 stars, based on 1 article reviews
Laforin Reactions, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00310/Uridine-5'-diphosphate+disodium+salt/pmc04843132-160-6-10
Average 95 stars, based on 1 article reviews
laforin reactions - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE"
Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE
Journal: Archives of biochemistry and biophysics
doi: 10.1016/j.abb.2016.03.020
Figure Legend Snippet: Effect of treating 32P-labeled glycogen with laforin or glucosidases. A Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.
Techniques Used: Labeling, Incubation, Mutagenesis, SDS Page
![Release of 32Pi from 32P-glycogen by laforin. Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (A) or [U-14C]glucose (B) and yeast glycogen synthase (5 μg/ml) for 30 min. Glycogen was precipitated three times with ethanol, treated with PiBind™ resin, purified by gel filtration and dissolved in buffer. The glycogen was incubated for 2 hr with 50 μg/ml laforin (WT Laf) or laforin inactivated by boiling for 5 min (HI Laf). A control (C) lacked laforin. The reaction mixtures were analyzed by TLC using PEI-cellulose plates. Standards of glucose-1-P (14C-G1P), glucose (14C-Glu) and inorganic phosphate (32Pi), labeled with the indicated isotope, were also analyzed.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3132/pmc04843132/pmc04843132__nihms-776793-f0005.jpg "Release of 32Pi from 32P-glycogen by laforin. Glycogen was labeled by incubation with 5 μM ...")
Figure Legend Snippet: Release of 32Pi from 32P-glycogen by laforin. Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (A) or [U-14C]glucose (B) and yeast glycogen synthase (5 μg/ml) for 30 min. Glycogen was precipitated three times with ethanol, treated with PiBind™ resin, purified by gel filtration and dissolved in buffer. The glycogen was incubated for 2 hr with 50 μg/ml laforin (WT Laf) or laforin inactivated by boiling for 5 min (HI Laf). A control (C) lacked laforin. The reaction mixtures were analyzed by TLC using PEI-cellulose plates. Standards of glucose-1-P (14C-G1P), glucose (14C-Glu) and inorganic phosphate (32Pi), labeled with the indicated isotope, were also analyzed.
Techniques Used: Labeling, Incubation, Purification, Filtration
Related Articles
Labeling:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. Incubation:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. Mutagenesis:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. SDS Page:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. Purification:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. Filtration:Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE Article Snippet: [α- 32 P]UDP (SCP230/37; specific activity 6,000 Ci/mmol in 50 mM Tricine pH 7.4) was from Hartmann Analytic. |