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detergent  (Chem Impex International)


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  • 95

    Structured Review

    Chem Impex International detergent
    Detergent, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/00234/n-Octyl-b-D-glucopyranoside/10__7554_slash_elife__68548-232-22-15
    Average 95 stars, based on 10 article reviews
    detergent - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Gut Microbial Metabolism of 5-ASA Diminishes Its Clinical Efficacy in Inflammatory Bowel Disease
    Article Snippet: These diluted cultures were grown anaerobically in sealed flasks at 37 °C to an OD 600 ~0.6 at which point protein expression was induced by the addition of 100 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG, TEKNOVA) followed by aerobic culture overnight at 16 °C. .. The following morning, E. coli were pelleted by centrifugation and then lysed in 20 mM HEPES pH 8.0 buffer containing 30 mM imidazole and 300 mM NaCl and supplemented with 0.5% Octyl-B-D-thrioglucopyranoside (Chem-Impex), 0.5 mg/mL lysozyme (Sigma), and SIGFAST protease inhibitor cocktail (Sigma). .. After lysis and clarification by centrifugation, lysates were incubated for 1 hour at 4 °C with His-Pure Cobalt Purification beads (Thermo).

    Article Title: Identification and targeting of microbial putrescine acetylation in bloodstream infections
    Article Snippet: These diluted cultures were grown shaking at 37 °C to an OD600 of ∼0.6, at which point protein expression was induced by the addition of 200 μM isopropyl β-D-1-thiogalactopyranoside (IPTG, Teknova), followed by culturing shaking overnight at 16 °C. .. The next morning, E. coli were pelleted by centrifugation and then lysed in 20 mM HEPES pH 8.0 buffer containing 30 mM imidazole and 300 mM NaCl and supplemented with 0.5% octyl-β-D-thrioglucopyranoside (Chem-Impex), 0.5 mg/mL lysozyme (Sigma-Aldrich) and SIGFAST protease inhibitor cocktail (Sigma-Aldrich). .. After lysis and clarification by centrifugation, lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific).

    Protease Inhibitor:

    Article Title: Gut Microbial Metabolism of 5-ASA Diminishes Its Clinical Efficacy in Inflammatory Bowel Disease
    Article Snippet: These diluted cultures were grown anaerobically in sealed flasks at 37 °C to an OD 600 ~0.6 at which point protein expression was induced by the addition of 100 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG, TEKNOVA) followed by aerobic culture overnight at 16 °C. .. The following morning, E. coli were pelleted by centrifugation and then lysed in 20 mM HEPES pH 8.0 buffer containing 30 mM imidazole and 300 mM NaCl and supplemented with 0.5% Octyl-B-D-thrioglucopyranoside (Chem-Impex), 0.5 mg/mL lysozyme (Sigma), and SIGFAST protease inhibitor cocktail (Sigma). .. After lysis and clarification by centrifugation, lysates were incubated for 1 hour at 4 °C with His-Pure Cobalt Purification beads (Thermo).

    Article Title: Identification and targeting of microbial putrescine acetylation in bloodstream infections
    Article Snippet: These diluted cultures were grown shaking at 37 °C to an OD600 of ∼0.6, at which point protein expression was induced by the addition of 200 μM isopropyl β-D-1-thiogalactopyranoside (IPTG, Teknova), followed by culturing shaking overnight at 16 °C. .. The next morning, E. coli were pelleted by centrifugation and then lysed in 20 mM HEPES pH 8.0 buffer containing 30 mM imidazole and 300 mM NaCl and supplemented with 0.5% octyl-β-D-thrioglucopyranoside (Chem-Impex), 0.5 mg/mL lysozyme (Sigma-Aldrich) and SIGFAST protease inhibitor cocktail (Sigma-Aldrich). .. After lysis and clarification by centrifugation, lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific).

    Molecular Weight:

    Article Title: Studying membrane protein-lipid specificity through direct native mass spectrometric analysis from tunable proteoliposomes
    Article Snippet: .. Materials All lipids used in this study were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). n-Octyl-β-D-glucopyranoside (OG) was purchased from CHEM-IMPEX INT’L INC. Spectra/Por 6–8 kD Molecular weight cut-off dialysis membrane was purchased from Fisher Scientific. .. Ammonium acetate solution was purchased from Millipore Sigma.

    Article Title: Studying membrane protein-lipid specificity through direct native mass spectrometric analysis from tunable proteoliposomes
    Article Snippet: Finally, all images were acquired using a JEOL JEM 1400-plus at 120 kV. .. All lipids used in this study were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). n-Octyl-β-D-glucopyranoside (OG) was purchased from CHEM-IMPEX INT’L INC. Spectra/Por 6–8 kD Molecular weight cut-off dialysis membrane was purchased from Fisher Scientific. .. Ammonium acetate solution was purchased from Millipore Sigma.

    Membrane:

    Article Title: Studying membrane protein-lipid specificity through direct native mass spectrometric analysis from tunable proteoliposomes
    Article Snippet: .. Materials All lipids used in this study were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). n-Octyl-β-D-glucopyranoside (OG) was purchased from CHEM-IMPEX INT’L INC. Spectra/Por 6–8 kD Molecular weight cut-off dialysis membrane was purchased from Fisher Scientific. .. Ammonium acetate solution was purchased from Millipore Sigma.

    Article Title: Studying membrane protein-lipid specificity through direct native mass spectrometric analysis from tunable proteoliposomes
    Article Snippet: Finally, all images were acquired using a JEOL JEM 1400-plus at 120 kV. .. All lipids used in this study were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). n-Octyl-β-D-glucopyranoside (OG) was purchased from CHEM-IMPEX INT’L INC. Spectra/Por 6–8 kD Molecular weight cut-off dialysis membrane was purchased from Fisher Scientific. .. Ammonium acetate solution was purchased from Millipore Sigma.

    other:

    Article Title: Barrel expansion of outer membrane protein G nanopore through β-hairpin duplication.
    Article Snippet: Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts, USA Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, Massachusetts, USA Department of Chemistry, University of Science, Vietnam National University, Hanoi, Vietnam Department of Life Sciences, Imperial College London, London, UK Department of Biomedical Engineering, University of Illinois Chicago, Chicago, Illinois, USA Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, UK



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