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tubulin antibody  (TaKaRa)


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    Structured Review

    TaKaRa tubulin antibody
    Tubulin Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0021vct/rLV%2EEF1%2EmCherry-Tubulin-9/pmc04311200-418-2-4
    Average 94 stars, based on 2 article reviews
    tubulin antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Soybean miR172c Targets the Repressive AP2 Transcription Factor NNC1 to Activate ENOD40 Expression and Regulate Nodule Initiation
    Article Snippet: .. GFP and tubulin antibody (Clontech) were used for immunoblot analysis. .. Immunoreactive proteins were detected using an ECL Plus Chemiluminescence Kit (GE Healthcare).

    Construct:

    Article Title: Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0x104 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5x105 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. DNA constructs, siRNA and transfection conditions. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0x106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Article Title: K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15
    Article Snippet: .. For time-lapse imaging of postablation NuMA recruitment in Kif15-inhibited cells, plates were cotransfected with mCherry-tubulin (human α-tubulin in pmCherry-C1, Takara Bio; constructed by Michael Davison; gift of Sophie Dumont) and GFP-NuMA (human NuMA in pEGFP-N1, Takara Bio; constructed by Duane Compton group [Kisurina-Evgenieva et al., 2004]; gift of Sophie Dumont) 48– 96 h prior to imaging live using ViaFect Transfection Reagent (Pro- mega; Elting et al., 2014). ..

    Article Title: Midbody remnant inheritance is regulated by the ESCRT subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0×10 4 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5×10 5 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0×10 6 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Expressing:

    Article Title: Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0x104 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5x105 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. DNA constructs, siRNA and transfection conditions. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0x106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Article Title: Midbody remnant inheritance is regulated by the ESCRT subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0×10 4 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5×10 5 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0×10 6 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Stable Transfection:

    Article Title: Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0x104 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5x105 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. DNA constructs, siRNA and transfection conditions. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0x106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Article Title: Midbody remnant inheritance is regulated by the ESCRT subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0×10 4 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5×10 5 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0×10 6 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Generated:

    Article Title: Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0x104 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5x105 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. DNA constructs, siRNA and transfection conditions. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0x106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Article Title: Midbody remnant inheritance is regulated by the ESCRT subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0×10 4 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5×10 5 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0×10 6 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Transfection:

    Article Title: Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0x104 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5x105 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. DNA constructs, siRNA and transfection conditions. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0x106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Article Title: K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15
    Article Snippet: .. For time-lapse imaging of postablation NuMA recruitment in Kif15-inhibited cells, plates were cotransfected with mCherry-tubulin (human α-tubulin in pmCherry-C1, Takara Bio; constructed by Michael Davison; gift of Sophie Dumont) and GFP-NuMA (human NuMA in pEGFP-N1, Takara Bio; constructed by Duane Compton group [Kisurina-Evgenieva et al., 2004]; gift of Sophie Dumont) 48– 96 h prior to imaging live using ViaFect Transfection Reagent (Pro- mega; Elting et al., 2014). ..

    Article Title: Midbody remnant inheritance is regulated by the ESCRT subunit CHMP4C
    Article Snippet: For immunofluorescence and quantitative analysis, 3.0×10 4 cells were plated onto coverslips maintained in 24-well multiwell plates and grown for 48 h. For correlative light and electron microscopy and time-lapse studies 1.5×10 5 cells were plated onto 35-mm glass-bottom plates (MatTek) and grown for 48 h. .. The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio, Inc. MDCK II cells stably expressing these proteins were generated by transfection of 1.0×10 6 cells with Amaxa Nucleofector II (Lonza) using the L-005 program. .. After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope.

    Imaging:

    Article Title: K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15
    Article Snippet: .. For time-lapse imaging of postablation NuMA recruitment in Kif15-inhibited cells, plates were cotransfected with mCherry-tubulin (human α-tubulin in pmCherry-C1, Takara Bio; constructed by Michael Davison; gift of Sophie Dumont) and GFP-NuMA (human NuMA in pEGFP-N1, Takara Bio; constructed by Duane Compton group [Kisurina-Evgenieva et al., 2004]; gift of Sophie Dumont) 48– 96 h prior to imaging live using ViaFect Transfection Reagent (Pro- mega; Elting et al., 2014). ..



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    https://www.bioz.com/product/0021vct/rLV%2EEF1%2EmCherry-Tubulin-9/10__1091_slash_mbc__e20___06___0426-163-14-19
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