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amcyan fluorescent protein  (TaKaRa)


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    Structured Review

    TaKaRa amcyan fluorescent protein
    Amcyan Fluorescent Protein, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0011vct/rLV%2EEF1%2EAmCyan1-9/pmc12286854-309-21-25
    Average 93 stars, based on 4 article reviews
    amcyan fluorescent protein - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Interferon-γ orchestrates leptomeningeal anti-tumour response
    Article Snippet: Mouse cancer cell lines generated in this study were engineered to constitutively express V5-tagged Firefly luciferase (pLenti-PGK-V5-Luc-Puro w543-1 , Addgene, 19360), gift from E. Campeau and P. Kaufman. .. Some LeptoM derivatives (LLC LeptoM, E0771 LeptoM, B16 LeptoM) used in the flow cytometry experiments were additionally engineered to constitutively express AmCyan fluorescent protein (pLV-EF1a-AmCyan1-IRES-Puro, Takara, 0039VCT). .. Lentiviral constructs for CRISPR–Cas9 editing in the pLV-hCas9:T2A:Bsd backbone were synthetized by VectorBuilder. sgRNA sequences expressed under the control of U6 promoter were as follows: sg lacz , TGCGAATACGCCCACGCGAT; sg Ifngr2 .1, TGGACCTCCGAAAAACATCT; sg Ifngr2 .2, AGGGAACCTCACTTCCAAGT; sg Ifngr2 .3, TCTGTGATGTCCGTACAGTT.

    Article Title: Interferon-γ orchestrates leptomeningeal anti-tumour response.
    Article Snippet: Mouse cancer cell lines generated in this study were engineered to constitutively express V5-tagged Firefly luciferase (pLenti-PGK-V5-Luc-Purow543-1, Addgene, 19360), gift from E. Campeau and P. Kaufman. .. Some LeptoM derivatives (LLC LeptoM, E0771 LeptoM, B16 LeptoM) used in the flow cytometry experiments were additionally engineered to constitutively express AmCyan fluorescent protein (pLV-EF1a-AmCyan1-IRES-Puro, Takara, 0039VCT). .. Lentiviral constructs for CRISPR–Cas9 editing in the pLV-hCas9:T2A:Bsd backbone were synthetized by VectorBuilder. sgRNA sequences expressed under the control of U6 promoter were as follows: sglacz, TGCGAATACGCCCACGCGAT; sgIfngr2.1, TGGACCTCC GAAAAACATCT; sgIfngr2.2, AGGGAACCTCACTTCCAAGT; sgIfngr2.3, TCTGTGATGTCCGTACAGTT.

    Article Title: Leptomeningeal anti-tumor immunity follows unique signaling principles
    Article Snippet: Mouse cancer cell lines generated in this study were engineered to constitutively express V5-tagged Firefly luciferase (pLenti-PGK-V5-Luc-Puro w543–1 , Addgene #19360), kind gift from Dr. Eric Campeau and Dr. Paul Kaufman. .. Some LeptoM derivatives (LLC LeptoM, E0771 LeptoM, B16 LeptoM) used in the flow cytometry experiments were additionally engineered to constitutively express AmCyan fluorescent protein (pLV-EF1a-AmCyan1-IRES-Puro, Takara #0039VCT). .. Lentiviral constructs for CRISPR-Cas9 editing in the pLV-hCas9:T2A:Bsd backbone were synthetized by VectorBuilder. sgRNA sequences expressed under the control of U6 promoter were as follows: sg LacZ - ‘TGCGAATACGCCCACGCGAT’, sg Ifngr2 #1 ‘TGGACCTCCGAAAAACATCT’, sg Ifngr2 #2 ‘AGGGAACCTCACTTCCAAGT’, sg Ifngr2 #3 ‘TCTGTGATGTCCGTACAGTT’.

    In Vitro:

    Article Title: Single-cell atlas of human liver development reveals pathways directing hepatic cell fates
    Article Snippet: .. To model hepatic stellate cells in vitro we used the LX2 cell line, which was modified to obtain a CFP reporter cell line (rLV.EF1.AmCyan1-9 [0039VCT] Clontech, Takara Bio Europe). .. The LX2 cell line was maintained in DMEM, high glucose, GlutaMAXTM Supplement (Thermo Fisher Scientific, 10566016) in presence of 5% FBS (SLS, SH30070.03) and 1% Pen/Strep.

    Modification:

    Article Title: Single-cell atlas of human liver development reveals pathways directing hepatic cell fates
    Article Snippet: .. To model hepatic stellate cells in vitro we used the LX2 cell line, which was modified to obtain a CFP reporter cell line (rLV.EF1.AmCyan1-9 [0039VCT] Clontech, Takara Bio Europe). .. The LX2 cell line was maintained in DMEM, high glucose, GlutaMAXTM Supplement (Thermo Fisher Scientific, 10566016) in presence of 5% FBS (SLS, SH30070.03) and 1% Pen/Strep.



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