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triton x 100  (Biotium)


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    Structured Review

    Biotium triton x 100
    Triton X 100, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/00095-t/ActinBrite+488-505+High+Affinity+Phalloidin+Conjugate%2C+50+U/pmc08270983-235-23-36
    Average 93 stars, based on 56 article reviews
    triton x 100 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Automated production of nerve repair constructs containing endothelial cell tube-like structures.
    Article Snippet: Accepted Manuscript is “the version of the article accepted for publication including all changes made as a result of the peer review process, and which may also include the addition to the article by IOP Publishing of a header, an article ID, a cover sheet and/or an ‘Accepted Manuscript’ watermark, but excluding any other editing, typesetting or other changes made by IOP Publishing and/or its licensors” This Accepted Manuscript is © 2024 The Author(s).. Published by IOP Publishing Ltd.

    Article Title: Exploiting dysregulated iron homeostasis to eradicate persistent high-grade serous ovarian cancer
    Article Snippet: .. At 24 h post-compound treatment, cells were fixed with 4% paraformaldehyde and stained with 2 μg/mL DAPI and 0.4 U/mL rhodamine-phalloidin (Biotium #00027) diluted in PBS + 0.3% Triton X-100. .. Plates were imaged on the CellInsight CX7 LZR platform (Thermo Fisher Scientific) at 10X magnification using the blue (405 nm) and red (561 nm) excitation lasers and BGRFRN emission filters.

    Article Title: Mechanoreceptors initiate innate immunity in response to microbial infections
    Article Snippet: .. Briefly, directly conjugated antibodies against CD45, CD11c, and CD31 were added to the washed slides in PBS containing 1% BSA and 0.3% Triton X-100, along with a nuclear stain (Hoechst 33342, 1:1000, 40046 Biotium) and a blocking agent (anti-CD16/32, BioLegend), for 1 hour in a humid chamber at 37°C. .. The slides were then mounted with Fluoromount-G (00-4958-02, Thermo Scientific) and imaged using a Thunder Imager DMi8 (Leica).

    Saline:

    Article Title: Aquaporin-4 mis-localization slows glymphatic clearance of α-synuclein and promotes α-synuclein pathology and aggregate propagation
    Article Snippet: Tissue was deparaffinized, followed by heated antigen retrieval in Tris-EDTA pH 9.0 with tween (Abcam recipe) in a steamer for 20 minutes, followed by further antigen retrieval with 90% formic acid for 4 minutes. .. Sections were permeabilized in 0.3% Triton X-100 in phosphate buffered saline (PBS) for 15 minutes, rinsed in PBS, and treated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, catalog# 23007) to quench lipofuscin and autofluorescence, followed by incubation with rabbit anti-p-S129 (10ug/mL; Abcam; catalog # ab51253) diluted in 5% normal donkey serum in phosphate-buffered saline overnight at 4°C. .. Following overnight primary incubation, sections were incubated with secondary antibody donkey anti-rabbit Alexa Fluor 647 (1:400; Invitrogen, catalog #A21207) for 2 hours at room temperature.

    Incubation:

    Article Title: Aquaporin-4 mis-localization slows glymphatic clearance of α-synuclein and promotes α-synuclein pathology and aggregate propagation
    Article Snippet: Tissue was deparaffinized, followed by heated antigen retrieval in Tris-EDTA pH 9.0 with tween (Abcam recipe) in a steamer for 20 minutes, followed by further antigen retrieval with 90% formic acid for 4 minutes. .. Sections were permeabilized in 0.3% Triton X-100 in phosphate buffered saline (PBS) for 15 minutes, rinsed in PBS, and treated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, catalog# 23007) to quench lipofuscin and autofluorescence, followed by incubation with rabbit anti-p-S129 (10ug/mL; Abcam; catalog # ab51253) diluted in 5% normal donkey serum in phosphate-buffered saline overnight at 4°C. .. Following overnight primary incubation, sections were incubated with secondary antibody donkey anti-rabbit Alexa Fluor 647 (1:400; Invitrogen, catalog #A21207) for 2 hours at room temperature.

    Lysis:

    Article Title: Design, Synthesis, and Pharmacological Evaluation of Dual FXR-LIFR Modulators for the Treatment of Liver Fibrosis.
    Article Snippet: Although multiple approaches have been suggested, treating mild-to-severe fibrosis in the context of metabolic dysfunction associated with liver disease (MASLD) remains a challenging area in drug discovery.. Pathogenesis of liver fibrosis is multifactorial, and pathogenic mechanisms are deeply intertwined; thus, it is well accepted that future treatment requires the development of multitarget modulators.. Harnessing the 3,4,5-trisubstituted isoxazole scaffold, previously described as a key moiety in Farnesoid X receptor (FXR) agonism, herein we report the discovery of a novel class of hybrid molecules endowed with dual activity toward FXR and the leukemia inhibitory factor receptor (LIFR).

    Luciferase:

    Article Title: Design, Synthesis, and Pharmacological Evaluation of Dual FXR-LIFR Modulators for the Treatment of Liver Fibrosis.
    Article Snippet: Although multiple approaches have been suggested, treating mild-to-severe fibrosis in the context of metabolic dysfunction associated with liver disease (MASLD) remains a challenging area in drug discovery.. Pathogenesis of liver fibrosis is multifactorial, and pathogenic mechanisms are deeply intertwined; thus, it is well accepted that future treatment requires the development of multitarget modulators.. Harnessing the 3,4,5-trisubstituted isoxazole scaffold, previously described as a key moiety in Farnesoid X receptor (FXR) agonism, herein we report the discovery of a novel class of hybrid molecules endowed with dual activity toward FXR and the leukemia inhibitory factor receptor (LIFR).

    Activity Assay:

    Article Title: Design, Synthesis, and Pharmacological Evaluation of Dual FXR-LIFR Modulators for the Treatment of Liver Fibrosis.
    Article Snippet: Although multiple approaches have been suggested, treating mild-to-severe fibrosis in the context of metabolic dysfunction associated with liver disease (MASLD) remains a challenging area in drug discovery.. Pathogenesis of liver fibrosis is multifactorial, and pathogenic mechanisms are deeply intertwined; thus, it is well accepted that future treatment requires the development of multitarget modulators.. Harnessing the 3,4,5-trisubstituted isoxazole scaffold, previously described as a key moiety in Farnesoid X receptor (FXR) agonism, herein we report the discovery of a novel class of hybrid molecules endowed with dual activity toward FXR and the leukemia inhibitory factor receptor (LIFR).

    Cell Culture:

    Article Title: An emulsion electrospun nanofibrous scaffold loaded with glial cell line-derived neurotrophic factor for nerve regeneration
    Article Snippet: .. Nanofibre squares with cultured cells were first submerged in 0.1% Sudan black B in 70% ethanol for 40 min, followed by 0.5% Triton X-100 in PBS for 20 min. Those laden with Schwann cells were then immersed overnight in 1:200 rhodamine-conjugated phalloidin (00032, Biotium) in PBS at 4°C. .. Nanofibres with cultured primary neurons were treated with 5% goat serum in PBS followed by 1:500 Alexa Fluor® 488-conjugated anti-βIII tubulin (ab195879, abcam) in 1% goat serum in PBS overnight at 4°C.

    Blocking Assay:

    Article Title: Mechanoreceptors initiate innate immunity in response to microbial infections
    Article Snippet: .. Briefly, directly conjugated antibodies against CD45, CD11c, and CD31 were added to the washed slides in PBS containing 1% BSA and 0.3% Triton X-100, along with a nuclear stain (Hoechst 33342, 1:1000, 40046 Biotium) and a blocking agent (anti-CD16/32, BioLegend), for 1 hour in a humid chamber at 37°C. .. The slides were then mounted with Fluoromount-G (00-4958-02, Thermo Scientific) and imaged using a Thunder Imager DMi8 (Leica).



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