chaps  (Chem Impex International)

Journal: bioRxiv

Article Title: Dendritic inhibition terminates plateau potentials in CA1 pyramidal neurons

doi: 10.1101/2025.06.05.657434

Figure Lengend Snippet: A , Plateaus recorded in control (top) and in the presence of TTX (1 µM). Action potentials and plateaus were initially abolished (middle). Increasing the current injection amplitude recovered the plateaus but not the action potentials (bottom). Examples show 5 consecutive traces. B , Just-suprathreshold current injection for neurons recorded in control and following bath application of TTX (top). Plateau duration in control and following recovery by increased depolarization in TTX (bottom). C , Plateaus recorded in the presence of TTX (black) and following the addition of CdCl 2 (100 µM, blue). 5 consecutive traces are shown for both. D , Plateau duration recorded in the presence of TTX and in the presence of TTX + CdCl 2 . E , Plateaus recorded in the presence of TTX (black) and following perfusion of TTX + apamin (APA, 100 nM, red). F , Plateau duration measured in TTX and following the application of TTX + APA. G , H , Plateaus were terminated by mock IPSPs in the presence of TTX (6/6 neurons) and in the presence of TTX + apamin (6/6 neurons). Examples show successive sweeps in which the mock IPSP amplitude was gradually increased. Dark lines show the just-subthreshold and just-suprathreshold trials for the V M recordings and the injected current.

Article Snippet: The following compounds were also used in experiments: TTX (1 µM, tetrodotoxin with citrate, Biotium), apamin (100 nM, Medchemexpress LLC) and CdCl 2 (100 µM, Thermo Scientific Chemicals).

Techniques: Control, Injection