Saline:Article Title: Dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline
Article Snippet: .. Following three rinses with Phosphate buffered saline (PBS), lumbrical muscles were dissected for staining with anti-SV2 (Developmental Studies Hybridoma Bank AB2315387, 1:200), anti-2H3 (Developmental Studies Hybridoma Bank AB2314897, 1:100) and α-bungarotoxin (BTX) (Biotium 00006, 1:500) as described previously . ..
Muscles:Article Title: Dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline
Article Snippet: .. Following three rinses with Phosphate buffered saline (PBS), lumbrical muscles were dissected for staining with anti-SV2 (Developmental Studies Hybridoma Bank AB2315387, 1:200), anti-2H3 (Developmental Studies Hybridoma Bank AB2314897, 1:100) and α-bungarotoxin (BTX) (Biotium 00006, 1:500) as described previously . ..
Staining:Article Title: Dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline
Article Snippet: .. Following three rinses with Phosphate buffered saline (PBS), lumbrical muscles were dissected for staining with anti-SV2 (Developmental Studies Hybridoma Bank AB2315387, 1:200), anti-2H3 (Developmental Studies Hybridoma Bank AB2314897, 1:100) and α-bungarotoxin (BTX) (Biotium 00006, 1:500) as described previously . ..
Article Title: Mannose Targeting and Hydrophobic Tuning of Polycationic Vectors for Efficient Immunostimulatory CpG Delivery
Article Snippet: Ovalbumin-encoding plasmid (pOVA) was purchased from GeneScript (Leiden, Netherlands) and amplified by Escherichia coli bacterial cell transformation using One Shot TOP10 chemically competent E. coli (Invitrogen, Carlsbad) and purified with a NucleoBond Xtra Maxi kit (Macherey-Nagel GmbH, Düren, Germany). .. GelRed nucleic acid staining solution 10,000× in water was purchased from Biotium (Fremont, CA). .. Purified anti-mouse CD16/32 antibody (clone 93), Alexa Fluor 647 anti-mouse CD206 Antibody, Alexa Fluor 647 Rat IgG2a, k Isotype Ctrl, and Alexa Fluor 647-conjugate goat anti-Rat IgG were purchased from Biolegend (San Diego, CA).
Article Title: Comparative Assessment of Viral Load Retention in Surgical and Fabric Masks Worn by COVID-19 Patients
Article Snippet: The final product (1583–1592 bp) was visualized on 1.5% agarose gel (Sigma-Aldrich, St. Louis, MO, USA) prepared in Tris-Borate-EDTA (TBE) buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3), electrophoresed at 80 W/h, and photo-documented using a transilluminator (STi gel, Loccus Biotecnologia, Diadema, SP, Brazil). .. The PCR product was diluted in bromophenol blue, stained with GelRed ® 10,000× (Biotium Inc., Fremont, CA, USA) at a working concentration of 20× (1:500) and estimated using a 1 kb Plus molecular marker (Invitrogen/Life Technologies, Carlsbad, CA, USA). .. Purification of the PCR product was conducted using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and quantification was estimated with a molecular low-mass marker (Invitrogen/Life Technologies, Carlsbad, CA, USA).
Amplification:
Isolation:
Electrophoresis:
Imaging:
Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Recombinant:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Modification:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Transfection:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Plasmid Preparation:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Software:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Cell Culture:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Microscopy:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Targeted Proteomics:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Mass Spectrometry:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Activation Assay:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Clinical Proteomics:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Membrane:Article Title: Differential increase in endocannabinoid levels at the plasma and intracellular membranes
Article Snippet: .. 484 o Code: See Key resources table 485 o All other items: None 486 STAR Methods 487 Key resources table 488 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetateacetoxymethyl ester (BAPTA-AM) Sigma-Aldrich A4926 2-Arachidonoyl Glycerol-d5 Cayman Chemical 362162 2-aminoethoxydiphenyl borate (2-APB) Tocris 1224 acetonitrile ThermoFisher Scientific A9551 bovine serum albumin Sigma-Aldrich A3803 bradykinin Sigma-Aldrich 05-23-0500 cadmium Sigma-Aldrich C3141 Cal-520 ATT Bioquest 21230 Dulbecco's Modified Eagles Medium (DMEM) ThermoFisher Scientific SH30243FS Jo urn al Pr e-p roo f 17 Dimethyl sulfoxide Sigma-Aldrich D2650 DO34 AOBIOUS AOB8060 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′tetraacetic acid (EGTA) Sigma-Aldrich E3889 ET-18-OCH3 R&D Systems Inc 7462 HOE 140 R&D Systems Inc A740003 mastoparan Sigma-Aldrich 444898 DPBS, no calcium, no magnesium ThermoFisher Scientific 14-190-250 Penicillin-Streptomycin (10,000 U/mL) ThermoFisher Scientific 15-140-122 pertussis Sigma-Aldrich P2980 pluronic acid F127 Biotium 59004 Poly-D-lysine Sigma-Aldrich P6407 Polyethylenimine, Linear, MW 25000, Transfection Grade Polysciences 23966 SR141716 NIDA Drug Supply thapsigargin R&D Systems Inc 1138 Trifluoroacetic acid ThermoFisher Scientific A11650 Trypsin-EDTA Sigma-Aldrich T4049 U73122 hydrate Sigma-Aldrich U6756 Wheat germ agglutinin Alexa Fluor 594 ThermoFisher Scientific W11262 Experimental models: Cell lines Neuro2a cells This lab GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Mut-GRABeCB2.0 in AM/CBA-WPRE-bGH plasmid This lab Software and algorithms Fiji https://imagej.net/ Prism GraphPad Matlab MathWorks Deposited data Code: available at https://github.com/Stell aLab/StellaLab.git See reference 16 Other 35 mm glass coverslip imaging cell culture dish Mattek P35G-1.5-10-C Confocal Microscope Leica SP8X Triple quadrupole mass spectrometer Waters Xevo TQ-S Zorbax C18 2.1 x 50 mm, 3.5 um reverse-phase column Agilent Fluorescent plate reader Molecular devices SpectraMax M2e 96-Well Optical-Bottom Microplate, black, TC surface ThermoFisher Scientific 165305 Experimental model and study participant details 489 Neuro2a cells can be maintained either as undifferentiated, proliferating cells or undergo differentiation 490 following the select activation of both plasma membrane and intracellular receptor targets, such as 491 CB1R and PPAR, respectively 75-77. ..
Polymerase Chain Reaction:Article Title: Comparative Assessment of Viral Load Retention in Surgical and Fabric Masks Worn by COVID-19 Patients
Article Snippet: The final product (1583–1592 bp) was visualized on 1.5% agarose gel (Sigma-Aldrich, St. Louis, MO, USA) prepared in Tris-Borate-EDTA (TBE) buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3), electrophoresed at 80 W/h, and photo-documented using a transilluminator (STi gel, Loccus Biotecnologia, Diadema, SP, Brazil). .. The PCR product was diluted in bromophenol blue, stained with GelRed ® 10,000× (Biotium Inc., Fremont, CA, USA) at a working concentration of 20× (1:500) and estimated using a 1 kb Plus molecular marker (Invitrogen/Life Technologies, Carlsbad, CA, USA). .. Purification of the PCR product was conducted using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and quantification was estimated with a molecular low-mass marker (Invitrogen/Life Technologies, Carlsbad, CA, USA).
Concentration Assay:Article Title: Comparative Assessment of Viral Load Retention in Surgical and Fabric Masks Worn by COVID-19 Patients
Article Snippet: The final product (1583–1592 bp) was visualized on 1.5% agarose gel (Sigma-Aldrich, St. Louis, MO, USA) prepared in Tris-Borate-EDTA (TBE) buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3), electrophoresed at 80 W/h, and photo-documented using a transilluminator (STi gel, Loccus Biotecnologia, Diadema, SP, Brazil). .. The PCR product was diluted in bromophenol blue, stained with GelRed ® 10,000× (Biotium Inc., Fremont, CA, USA) at a working concentration of 20× (1:500) and estimated using a 1 kb Plus molecular marker (Invitrogen/Life Technologies, Carlsbad, CA, USA). .. Purification of the PCR product was conducted using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and quantification was estimated with a molecular low-mass marker (Invitrogen/Life Technologies, Carlsbad, CA, USA).
Marker:Article Title: Comparative Assessment of Viral Load Retention in Surgical and Fabric Masks Worn by COVID-19 Patients
Article Snippet: The final product (1583–1592 bp) was visualized on 1.5% agarose gel (Sigma-Aldrich, St. Louis, MO, USA) prepared in Tris-Borate-EDTA (TBE) buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3), electrophoresed at 80 W/h, and photo-documented using a transilluminator (STi gel, Loccus Biotecnologia, Diadema, SP, Brazil). .. The PCR product was diluted in bromophenol blue, stained with GelRed ® 10,000× (Biotium Inc., Fremont, CA, USA) at a working concentration of 20× (1:500) and estimated using a 1 kb Plus molecular marker (Invitrogen/Life Technologies, Carlsbad, CA, USA). .. Purification of the PCR product was conducted using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and quantification was estimated with a molecular low-mass marker (Invitrogen/Life Technologies, Carlsbad, CA, USA).
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